Nagai Tomoaki, Mizuno Kensaku
Laboratory of Molecular Cell Biology, Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
J Biochem. 2014 Mar;155(3):137-46. doi: 10.1093/jb/mvu001. Epub 2014 Jan 7.
Furry (Fry) is a large protein that is evolutionarily conserved from yeast to human. Fry and its orthologues in invertebrates (termed Tao3p in budding yeast, Mor2p in fission yeast, Sax-2 in nematode and Fry in fruit fly) genetically and physically interact with nuclear Dbf2-related (NDR) kinases (termed Cbk1p in budding yeast, Orb6p in fission yeast, Sax-1 in nematode and Trc in fruitfly), and function as activators or scaffolds of these kinases. Fry-NDR kinase signals are implicated in the control of polarized cell growth and morphogenesis in yeast, neurite outgrowth in nematode, and epidermal morphogenesis and dendritic tiling in fruit fly. Recent studies revealed that mammalian Fry is a microtubule-associated protein that is involved in the control of chromosome alignment, spindle organization and Polo-like kinase-1 activation in mitosis, and promotes microtubule acetylation in mitotic spindles via inhibiting the tubulin deacetylase Sirtuin 2. Here, we review current knowledge about the diverse cellular functions and regulation of Fry proteins in invertebrates and vertebrates.
Furry（Fry）是一种大型蛋白质，从酵母到人类在进化上高度保守。Fry及其在无脊椎动物中的同源物（在芽殖酵母中称为Tao3p，在裂殖酵母中称为Mor2p，在线虫中称为Sax - 2，在果蝇中称为Fry）与核Dbf2相关（NDR）激酶（在芽殖酵母中称为Cbk1p，在裂殖酵母中称为Orb6p，在线虫中称为Sax - 1，在果蝇中称为Trc）在遗传和物理上相互作用，并作为这些激酶的激活剂或支架发挥作用。Fry - NDR激酶信号参与酵母中极化细胞生长和形态发生的控制、线虫中的神经突生长以及果蝇中的表皮形态发生和树突平铺。最近的研究表明，哺乳动物的Fry是一种微管相关蛋白，参与有丝分裂中染色体排列、纺锤体组织和Polo样激酶 - 1激活的控制，并通过抑制微管去乙酰化酶Sirtuin 2促进有丝分裂纺锤体中的微管乙酰化。在这里，我们综述了关于Fry蛋白在无脊椎动物和脊椎动物中不同细胞功能和调控的现有知识。
