杂交的新概念：在应用粘结系统之前，用菠萝蛋白酶对牙本质进行脱蛋白处理。
A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system.
作者信息
Dayem Raad Niama, Tameesh Mona Adnan
机构信息
Professor Dr. Raad Niama Dayem BDS, MSc, PhD Conservative Dentistry, USA.
出版信息
Contemp Clin Dent. 2013 Oct;4(4):421-6. doi: 10.4103/0976-237X.123015.
OBJECTIVE
The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope.
MATERIALS AND METHODS
A total of 60 extracted human upper premolars were selected to be given standardized buccal and lingual class V cavities. The teeth were divided into three groups each one consisted of 20 teeth. Thirty teeth were recruited for SEM study and the other 30 for polarized microscope. Group 1: Teeth were deproteinized with Nd:YAG laser, Group 2: Teeth were deproteinized with bromelain enzyme and Group 3: Teeth were deproteinized with 10% NaOCl.
RESULTS AND CONCLUSIONS
Application of bromelain enzyme has led to removing collagen network and significantly decreased the global leakage scores of the adhesive system.
目的
本研究的目的是通过扫描电子显微镜（SEM）和偏光显微镜评估菠萝蛋白酶的脱蛋白效果，并将其与掺钕钇铝石榴石（Nd:YAG）激光和10%次氯酸钠（NaOCl）进行比较。
材料与方法
总共选择60颗拔除的人类上颌前磨牙，制备标准化的颊侧和舌侧V类洞。将牙齿分为三组，每组20颗。30颗牙齿用于SEM研究，另外30颗用于偏光显微镜研究。第1组：用Nd:YAG激光对牙齿进行脱蛋白处理；第2组：用菠萝蛋白酶对牙齿进行脱蛋白处理；第3组：用10% NaOCl对牙齿进行脱蛋白处理。
结果与结论
菠萝蛋白酶的应用导致胶原网络的去除，并显著降低了粘结系统的整体渗漏评分。
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