采用与微针阵列集成的光流控装置对生物分子进行电动捕获和表面增强拉曼散射检测。
Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array.
机构信息
Department of Chemical Engineering, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan ; Center for Micro/Nano Science and Technology, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan ; Research Center for Energy Technology and Strategy, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan.
出版信息
Biomicrofluidics. 2013 Feb 21;7(1):14111. doi: 10.1063/1.4793224. eCollection 2013.
In this study, microneedles which possess sharp tips were utilized to trap and detect the biomolecules. Owing to the large curvature, the tips of the microneedles created a substantially high gradient of electric field under the non-uniform electric field which served as not only the trapping sites but also the substrate for surface enhanced Raman scattering (SERS). Separation of polystyrene microparticles with different sizes and two kinds of biomolecules (Staphylococcus aureus (S. aureus) and the red blood cells (RBCs)) were demonstrated. Moreover, in situ detection of S. aureus was performed immediately after separation was completed. The results showed that, after 15 s of sample collection, the Raman signals of S. aureus were detected and greatly enhanced through SERS effect.
在这项研究中，使用了具有锋利尖端的微针来捕获和检测生物分子。由于曲率较大，微针的尖端在非均匀电场下产生了相当高的电场梯度，不仅作为捕获位点，而且还作为表面增强拉曼散射（SERS）的基底。展示了不同大小的聚苯乙烯微球和两种生物分子（金黄色葡萄球菌（金黄色葡萄球菌）和红细胞（RBC））的分离。此外，在分离完成后立即进行了金黄色葡萄球菌的原位检测。结果表明，在收集样品 15 秒后，通过 SERS 效应检测到并大大增强了金黄色葡萄球菌的拉曼信号。
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