云杉卷叶蛾（昆虫纲）培养细胞中对福斯高林不敏感的腺苷酸环化酶

Forskolin-insensitive adenylate cyclase in cultured cells of Choristoneura fumiferana (Insecta).

作者信息

Gole J W, Orr G L, Downer R G

出版信息

Biochem Biophys Res Commun. 1987 Jun 30;145(3):1192-7. doi: 10.1016/0006-291x(87)91563-4.

Abstract

Adenylate cyclase from a spruce budworm cell line, IBRI-Cfl, is activated by octopamine (Ka = 50 microM), guanine nucleotides and sodium fluoride but not by forskolin. In addition, forskolin does not potentiate the octopamine-sensitive response. To our knowledge, this is the first published report of a hormone-sensitive adenylate cyclase, with a functional guanine nucleotide regulatory protein, that is insensitive to forskolin both in intact cells and washed membrane preparations.

摘要

来自云杉芽虫细胞系IBRI - Cfl的腺苷酸环化酶可被章鱼胺（Ka = 50微摩尔）、鸟嘌呤核苷酸和氟化钠激活，但不能被福斯高林激活。此外，福斯高林不能增强对章鱼胺敏感的反应。据我们所知，这是首次发表的关于一种激素敏感的腺苷酸环化酶的报告，该酶具有功能性鸟嘌呤核苷酸调节蛋白，在完整细胞和洗涤后的膜制剂中均对福斯高林不敏感。