在32P磷酸盐的液体闪烁计数中遇到的化学发光效应。
A chemiluminescent effect encountered in the liquid scintillation counting of 32P phosphate.
作者信息
Bell T K, Lappin T R
出版信息
Ann Clin Biochem. 1978 Jan;15(1):37-9. doi: 10.1177/000456327801500110.
PMID:24405
Abstract
During the course of a kinetic enzyme study of bisphosphoglyceromutase (EC 2.7.5.4.) a chemiluminescent effect was encountered in the liquid scintillation counting of the beta-emission from 32Pi. A method is described for overcoming the effect, thereby permitting the accurate determination of the radioactive compounds. It is important to check for chemiluminescent effects early in the development of new procedures that involve liquid scintillation counting.
摘要
在对二磷酸甘油酸变位酶（EC 2.7.5.4.）进行动力学酶研究的过程中，在对³²P₁的β发射进行液体闪烁计数时遇到了化学发光效应。本文描述了一种克服该效应的方法，从而能够准确测定放射性化合物。在涉及液体闪烁计数的新程序开发早期检查化学发光效应很重要。
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