谷胱甘肽还原酶敲低对人肺腺癌细胞中 UVB 诱导的氧化应激的反应。
Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
机构信息
Institute of Bioinformatics and Structural Biology & Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.
出版信息
Proteome Sci. 2014 Jan 9;12(1):2. doi: 10.1186/1477-5956-12-2.
BACKGROUND
Glutathione reductase (GR) plays a critical role in the maintenance of physiological redox status in cells. However, the comprehensive investigations of GR-modulated oxidative stress have not been reported.
METHODS
In the present study, we cultured a human lung adenocarcinoma line CL1-0 and its GR-knockdown derivative CL1-0ΔGR to evaluate their differential responses to UVB-irradiation.
RESULTS
We identified 18 proteins that showed significant changes under UVB-irradiation in CL1-0ΔGR cells rather than in CL1-0 cells. Several proteins involving protein folding, metabolism, protein biosynthesis and redox regulation showed significant changes in expression.
CONCLUSIONS
In summary, the current study used a comprehensive lung adenocarcinoma-based proteomic approach for the identification of GR-modulated protein expression in response to UVB-irradiation. To our knowledge, this is the first global proteomic analysis to investigate the role of GR under UVB-irradiation in mammalian cell model.
背景
谷胱甘肽还原酶（GR）在维持细胞生理氧化还原状态方面发挥着关键作用。然而，GR 调节氧化应激的综合研究尚未见报道。
方法
本研究培养了人肺腺癌细胞系 CL1-0 及其 GR 敲低衍生物 CL1-0ΔGR，以评估它们对 UVB 照射的不同反应。
结果
我们鉴定出在 CL1-0ΔGR 细胞而非 CL1-0 细胞中，UVB 照射下有 18 种蛋白表达发生显著变化。涉及蛋白质折叠、代谢、蛋白质生物合成和氧化还原调节的几种蛋白质的表达发生了显著变化。
结论
总之，本研究使用基于肺腺癌的综合蛋白质组学方法，鉴定了 GR 调节的蛋白表达对 UVB 照射的反应。据我们所知，这是首次在哺乳动物细胞模型中进行的针对 GR 在 UVB 照射下作用的全球蛋白质组学分析。
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