来自酿酒酵母的一种必需小核仁RNA具有预测的U4的特性，包括与一种类似U6的小核仁RNA相互作用。

An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA.

作者信息

Siliciano P G, Brow D A, Roiha H, Guthrie C

出版信息

Cell. 1987 Aug 14;50(4):585-92. doi: 10.1016/0092-8674(87)90031-6.

Abstract

Three yeast snRNAs (snR20, snR7, and snR14) have been implicated in pre-mRNA splicing. snR20 and snR7 contain domains of homology to U2 and U5, respectively, and each is required for viability. These RNAs are found associated with the spliceosome, as is snR14. We show here that snR14 is also an essential gene product. Sequence analysis reveals that, like snR7 and snR20, snR14 contains a consensus binding site for the Sm antigen, a feature common to all mammalian snRNAs involved in splicing. Moreover, snR14 exhibits several blocks of sequence and structural homology to U4, which in metazoans is found in association with U6. Native gel electrophoresis demonstrates that snR14 is in fact base-paired with another yeast snRNA, designated snR6, which has primary sequence homology to U6. We conclude that snR14 is the yeast analog of U4.

摘要

三种酵母小核RNA（snR20、snR7和snR14）与前体mRNA剪接有关。snR20和snR7分别含有与U2和U5同源的结构域，且二者都是细胞存活所必需的。这些RNA与剪接体相关，snR14也是如此。我们在此表明，snR14也是一种必需的基因产物。序列分析显示，与snR7和snR20一样，snR14含有Sm抗原的共有结合位点，这是所有参与剪接的哺乳动物小核RNA的共同特征。此外，snR14与U4存在多个序列和结构同源区域，在多细胞动物中，U4与U6结合存在。非变性凝胶电泳表明，snR14实际上与另一种酵母小核RNA碱基配对，该RNA命名为snR6，其一级序列与U6同源。我们得出结论，snR14是U4的酵母类似物。