Green K, Berdecia R, Cheeks L
Curr Eye Res. 1987 Jun;6(6):835-8. doi: 10.3109/02713688709034851.
Mussel Adhesive Protein (MAP) was applied to either denuded rabbit cornea, or dialysis membrane with a 50,000 dalton molecular weight cut-off. The permeability of corneal endothelium or bare stroma was compared with demambranized stroma coated on one surface with MAP. The permeability of dialysis membrane, both in the presence and absence of MAP, was also determined to inulin (5,000 daltons) and dextran (40,000 daltons). Corneal endothelial permeability was much less than that of bare stroma, and coating denuded stroma with MAP on one surface had no effect on stromal permeability. Dialysis membrane permeability was not reduced after coating with different thickness of MAP, indicating that MAP offers little resistance to the passage of even large non-electrolytes and can serve as a basement membrane for cellular or tissue attachment without impairment of nutritional supply to the overlying cells.
贻贝粘附蛋白（MAP）被应用于去上皮的兔角膜或截留分子量为50,000道尔顿的透析膜。将角膜内皮或裸露基质的通透性与在一个表面涂有MAP的脱膜基质进行比较。还测定了在有和没有MAP存在的情况下透析膜对菊粉（5,000道尔顿）和葡聚糖（40,000道尔顿）的通透性。角膜内皮的通透性远低于裸露基质，并且在一个表面用MAP包被去上皮的基质对基质通透性没有影响。用不同厚度的MAP包被后，透析膜的通透性没有降低，这表明MAP对即使是大的非电解质的通过也几乎没有阻力，并且可以作为细胞或组织附着的基底膜，而不会损害对覆盖细胞的营养供应。
