Poggi A, Bottino C, Zocchi M R, Pantaleo G, Ciccone E, Mingari C, Moretta L, Moretta A
Eur J Immunol. 1987 Jul;17(7):1065-8. doi: 10.1002/eji.1830170725.
We have applied two-color fluorescence cytofluorometric techniques to the analysis of the distribution of T44 and CD3 antigens in peripheral blood human lymphocytes. While most CD3+ cells co-expressed T44 antigen, a small distinct subset was CD3+ T44- (2-10% of CD3+ cells). This cell subset also did not react with the WT31 monoclonal antibody (mAb), specific for an alpha/beta framework determinant of the T cell receptor (TCR). Lack of T44 antigen expression was also observed in purified CD3+ WT31- polyclonal populations that had been cultured in medium containing interleukin 2 (IL2) and as well as greater than 30 clones expressing the CD3+4-8-WT31- surface phenotype. Immunoprecipitation experiments confirmed that expression of T44 molecules was confined to CD3+ WT31+ peripheral blood T cells. While conventional CD3+ WT31+ cells produced IL2 in response to mAb directed to CD2, CD3 or T44 surface molecules, CD3+ WT31- cells did not respond to anti-T44 mAb but released IL2 following stimulation with anti-CD2 or anti-CD3 mAb. Therefore, assuming that anti-T44 mimicks the effect of a still undefined natural ligand our data suggest that T cells expressing the gamma-gene surface product may be signalled by stimuli which differ, at least in part, from those acting on CD3+ WT31+ T lymphocytes.
我们已应用双色荧光细胞荧光测定技术来分析外周血人淋巴细胞中T44和CD3抗原的分布。虽然大多数CD3+细胞共表达T44抗原，但有一个小的独特亚群是CD3+ T44-（占CD3+细胞的2%-10%）。该细胞亚群也不与WT31单克隆抗体（mAb）反应，WT31单克隆抗体对T细胞受体（TCR）的α/β框架决定簇具有特异性。在含有白细胞介素2（IL2）的培养基中培养的纯化CD3+ WT31-多克隆群体以及超过30个表达CD3+4-8-WT31-表面表型的克隆中也观察到缺乏T44抗原表达。免疫沉淀实验证实T44分子的表达仅限于CD3+ WT31+外周血T细胞。虽然传统的CD3+ WT31+细胞在针对CD2、CD3或T44表面分子的单克隆抗体刺激下产生IL2，但CD3+ WT31-细胞对抗T44单克隆抗体无反应，但在抗CD2或抗CD3单克隆抗体刺激后释放IL2。因此，假设抗T44模拟了一种尚未明确的天然配体的作用，我们的数据表明，表达γ基因表面产物的T细胞可能受到至少部分不同于作用于CD3+ WT31+ T淋巴细胞的刺激的信号传导。
