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利用质谱碎片法测定蓖麻(Ricinus communis L.)木质部汁液中的吲哚-3-乙酸。

Determinations of indole-3-acetic acid in xylem sap of Ricinus communis L. using mass fragmentography.

机构信息

School of Biological Sciences, University of Sussex, Falmer, BN1 9QG, Brighton, UK.

出版信息

Planta. 1979 Jan;144(3):299-303. doi: 10.1007/BF00388773.

DOI:10.1007/BF00388773
PMID:24407262
Abstract

Mass fragmentography employing a deuterated internal standard was used to make quantitative analyses of indole-3-acetic acid in xylem sap collected from Ricinus communis L. When contamination of the sap by microorganisms was reduced by frequent collection, levels of IAA were found to be less than 0.5 ng ml(-1). It is therefore proposed that the transpiration stream does not play a significant role in the transport of IAA within the plant.

摘要

采用氘化内标物的质量碎片分析,对从大花葵收集的木质部汁液中的吲哚-3-乙酸进行定量分析。当通过频繁采集减少汁液中微生物的污染时,发现 IAA 的水平小于 0.5ng/ml。因此,提出蒸腾流在植物内 IAA 的运输中不起重要作用。

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引用本文的文献

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本文引用的文献

1
The influence of epiphytic bacteriae on auxin metabolism.附生细菌对生长素代谢的影响。
Planta. 1966 Dec;68(4):327-34. doi: 10.1007/BF00386332.
2
Indolyl-3-acetic acid in cap and apex of maize roots: Identification and quantification by mass fragmentography.玉米根冠和根尖中的吲哚乙酸:用质谱碎片图法鉴定和定量。
Planta. 1974 Jan;120(2):107-12. doi: 10.1007/BF00384920.
3
Identification of IAA in phloem and root pressure saps of Ricinus communis L. by mass spectrometry.利用质谱法鉴定蓖麻(Ricinus communis L.)韧皮部和根压汁液中的吲哚乙酸。
Planta. 1974 Sep;119(3):257-61. doi: 10.1007/BF00429049.
4
Gas chromatography-mass spectrometric determinations of abscisic acid levels in the cap and the apex of maize roots.采用气相色谱-质谱法测定玉米根冠和根尖中的脱落酸水平。
Planta. 1977 Jan;134(1):23-7. doi: 10.1007/BF00390089.
5
Identification and measurement of indoleacetic and abscisic acids in the cambial region of Picea sitchensis (Bong.) Carr. by combined gas chromatography-mass spectrometry.利用气相色谱-质谱联用技术鉴定和测定云杉(Bong.)Carr.形成层区域中的吲哚乙酸和脱落酸。
Planta. 1978 Jan;139(2):133-8. doi: 10.1007/BF00387138.
6
A DESEEDED AVENA TEST METHOD FOR SMALL AMOUNTS OF AUXIN AND AUXIN PRECURSORS.矮燕麦赤霉酸测试法用于检测少量的生长素和生长素前体。
J Gen Physiol. 1937 Jan 20;20(3):311-34. doi: 10.1085/jgp.20.3.311.
7
Effect of the Roots on the Production of Auxin by the Coleoptile.根对胚芽鞘生长素产生的影响。
Proc Natl Acad Sci U S A. 1937 May;23(5):272-6. doi: 10.1073/pnas.23.5.272.
8
Quantitative aspects of urinary indolo-3-acetic acid and 5-hydroxyindole-3-acetic acid excretion.尿中吲哚 - 3 - 乙酸和5 - 羟吲哚 - 3 - 乙酸排泄的定量研究
J Chromatogr. 1975 Jun 18;109(2):436-8. doi: 10.1016/s0021-9673(01)91824-2.
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The occurrence of raphanatin as an endogenous cytokinin in radish seed. Identification and quantitation by gas chromatographic--mass spectrometric analysis using deuterium-labelled standards.萝卜种子中萝卜苷作为内源性细胞分裂素的存在。使用氘标记标准品通过气相色谱 - 质谱分析进行鉴定和定量。
FEBS Lett. 1977 Oct 15;82(2):211-4. doi: 10.1016/0014-5793(77)80586-3.