EMS、DES 和γ射线在水稻中的诱变效率和效果。

Mutagenic effectiveness and efficiency of EMS, DES and gamma-rays in rice.

机构信息

Kurukshetra University, Kurukshetra, India.

出版信息

Theor Appl Genet. 1977 Sep;50(5):241-6. doi: 10.1007/BF00273758.

Abstract

Data on chlorophyll mutation frequency after treatment with EMS, DES and gamma-rays and sequential administration of gamma-rays and the two alkylating agents in three varieties of rice have been used to work out quantitatively the effectiveness and efficiency of each mutagen and combination treatment. For effectiveness, the order is EMS > DES and for efficiency it is EMS > DES > gamma-rays. In some sequential treatments (Gamma-rays + DES in 'IR8' and 'Basmati'; DES + gamma-rays in 'IR8' and 'Jhona'; Gamma-rays + EMS in 'IR8' and 'Basmati'; and EMS + gamma-rays in 'IR8', 'Jhona' and 'Basmati') mutation frequency is more than additive (synergistic) but these treatments are decisively less efficient because of their relatively high injurious effects in the M1. generation. EMS induces more albinas than gamma-rays do. The mutational spectrum patterns induced by gamma-rays and DES are alike. In general, combination treatments tend to increase the frequency of albinas over other types of chlorophyll mutants.

摘要

用 EMS、DES 和 γ 射线处理以及三种水稻品种中 γ 射线和两种烷化剂的顺序处理后，叶绿素突变频率的数据已被用于定量计算每种诱变剂和组合处理的有效性和效率。对于有效性，顺序是 EMS > DES；对于效率，顺序是 EMS > DES > γ 射线。在一些序贯处理中（IR8 和 Basmati 中的γ射线+DES；IR8 和 Jhona 中的 DES+γ射线；IR8 和 Basmati 中的γ射线+EMS；以及 IR8、Jhona 和 Basmati 中的 EMS+γ射线），突变频率超过了相加（协同）的水平，但由于它们在 M1 代中相对较高的伤害效应，这些处理的效率较低。EMS 诱导的白化体比 γ 射线多。γ 射线和 DES 诱导的突变谱模式相似。一般来说，组合处理往往会增加白化体相对于其他类型叶绿素突变体的频率。