Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, 94720, Berkeley, CA, USA.
Planta. 1979 Jan;144(2):193-200. doi: 10.1007/BF00387270.
The conversion of U-labelled [(14)C]glucose-6-phosphate into other products by a soluble fraction of lysed spinach chloroplasts has been studied. It was found that both an oxidative pentose phosphate cycle and a glycolytic reaction sequence occur in this fraction. The formation of bisphosphates and of triose phosphates was ATP-dependent and occurred mainly via a glycolytic reaction sequence including a phosphofructokinase step. The conversion, of glucose-6-phosphate via the oxidative pentose phosphate cycle stopped with the formation of pentose monophosphates. This was found not to be because of a lack in transaldolase (or transketolase) activity, but because of the high concentration ratios of hexose monophosphate/pentose monophosphate used in our experiments for simulating the conditions in whole chloroplasts in the dark. Some regulatory properties of both the oxidative pentose phosphate cycle and of the glycolytic pathway were studied.
已研究过溶胀菠菜叶绿体裂解物中的可溶性部分将 U 标记的 [(14)C]葡萄糖-6-磷酸转化为其他产物。结果发现，该部分既存在氧化戊糖磷酸循环，也存在糖酵解反应序列。二磷酸和三磷酸糖的形成依赖于 ATP，主要通过包括磷酸果糖激酶步骤的糖酵解反应序列发生。通过氧化戊糖磷酸循环将葡萄糖-6-磷酸转化，其产物为戊糖单磷酸。这并不是因为转醛醇酶（或转酮醇酶）活性缺乏，而是因为我们在实验中使用了高浓度的己糖单磷酸/戊糖单磷酸比值，以模拟黑暗中整个叶绿体的条件。还研究了氧化戊糖磷酸循环和糖酵解途径的一些调节特性。
