Aziz Abdul, Sarwar Raju Golam, Das Abhijit, Ahmed Jamiuddin, Moghal Md Mizanur Rahman
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Adv Pharm Bull. 2014;4(1):15-9. doi: 10.5681/apb.2014.003. Epub 2013 Dec 23.
Crinum latifolium is a widely used plant in Asian folk and traditional medicine.In the present study, we have tried to find out the anthelmintic activity, total phenolic contents and cytotoxicity of the methanolic extract of the target plant.
Anthelmintic activity was assessed applying five different concentrations of the plant extract and recording the time of paralysis and death. Total phenolic contents were determined using Folin-Ciocaltu method, using Gallic acid as standard; while brine shrimp lethality test (BSLT) method was used to evaluate the cytotoxicity of the plant extract, where vincristine sulphate and DMSO was used as positive and negative control respectively.
The lowest time for paralysis and death of worms, for test sample at highest concentration (50mg/ml), were found 24±0.45 and 46.4±0.60 min respectively, which gradually increased with the decrease of concentration. On the other hand, albendazole, which was used as standard, caused paralysis and death of worms at 56.2±0.20 min and 77.4±0.24 min respectively; whereas no mortality of the worms was observed, when distilled water was used as control. The crude methanolic extract exhibited lower amount of total phenolic content (17.50±2.64 mg/ml). In case of cytotoxicity measurement, the crude methanolic extract showed positive result (with LC50 15.652 µg/ml) compared to standard Vincristine sulphate (0.839 µg/ml); which indicated that the leaves of Crinum latifolium possess mild cytotoxic principles.
Therefore, further studies are suggested to evaluate the possible mechanism of action and the active compounds responsible for the biological activities of the plant extract.
宽叶文殊兰是一种在亚洲民间和传统医学中广泛使用的植物。在本研究中，我们试图探究该目标植物甲醇提取物的驱虫活性、总酚含量和细胞毒性。
通过应用五种不同浓度的植物提取物并记录麻痹和死亡时间来评估驱虫活性。使用福林 - 西奥卡试剂法测定总酚含量，以没食子酸为标准；而采用卤虫致死试验（BSLT）法评估植物提取物的细胞毒性，分别使用硫酸长春新碱和二甲基亚砜作为阳性和阴性对照。
在最高浓度（50mg/ml）下，测试样品使蠕虫麻痹和死亡的最短时间分别为24±0.45分钟和46.4±0.60分钟，随着浓度降低而逐渐增加。另一方面，作为标准的阿苯达唑分别在56.2±0.20分钟和77.4±0.24分钟导致蠕虫麻痹和死亡；而当使用蒸馏水作为对照时，未观察到蠕虫死亡。粗甲醇提取物的总酚含量较低（17.50±2.64mg/ml）。在细胞毒性测定中，与标准硫酸长春新碱（0.839μg/ml）相比，粗甲醇提取物显示出阳性结果（LC50为15.652μg/ml）；这表明宽叶文殊兰的叶子具有温和的细胞毒性成分。
因此，建议进一步研究以评估植物提取物生物活性的可能作用机制和活性化合物。
