Wiederer O, Löffler G
J Lipid Res. 1987 Jun;28(6):649-58.
A reproducible cell culture system is described that allows the study of adipose conversion in fibroblast-like cells isolated by collagenase digestion of epididymal and perirenal adipose tissue from male rats weighing 70-200 g. Adipose conversion as measured by lipid accumulation and increase in glycerophosphate dehydrogenase (GPDH) activity during differentiation strongly depends on the density at which cells are inoculated and starts only when cells are confluent and when physiological amounts of corticosterone and insulin are added. beta-Estradiol, testosterone, thyroxine, triiodothyronine, and growth hormone do not affect the differentiation process. Methylisobutylxanthine added during the first 2 days after confluence, added with insulin and corticosterone, potentiates the effect of insulin on GPDH activity and accelerates triglyceride accumulation. The effect of methyl-isobutylxanthine seems to be mediated by increased cyclic AMP concentrations, inasmuch as it may be replaced by forskolin.
本文描述了一种可重复的细胞培养系统，该系统可用于研究从体重70 - 200克雄性大鼠的附睾和肾周脂肪组织经胶原酶消化分离出的成纤维细胞样细胞中的脂肪转化。在分化过程中，通过脂质积累和甘油磷酸脱氢酶（GPDH）活性增加来衡量的脂肪转化强烈依赖于接种细胞的密度，并且仅在细胞汇合以及添加生理量的皮质酮和胰岛素时才开始。β-雌二醇、睾酮、甲状腺素、三碘甲状腺原氨酸和生长激素不影响分化过程。在汇合后的前两天添加甲基异丁基黄嘌呤，并与胰岛素和皮质酮一起添加，可增强胰岛素对GPDH活性的作用并加速甘油三酯积累。甲基异丁基黄嘌呤的作用似乎是由环磷酸腺苷浓度增加介导的，因为它可以被福司可林替代。
