Ye Fei, Yu Hai-Li, Li Peng-Fei, You Ping
a College of Life Science, Shaanxi Normal University , Xi'an , China and.
b College of Life Science, Northwest University , Xi'an , China.
Mitochondrial DNA. 2015;26(6):853-4. doi: 10.3109/19401736.2013.861427. Epub 2014 Jan 10.
We have determined the complete mitochondrial genome of Lista haraldusalis Walker, 1859. The mitochondrial genome of L. haraldusalis is 15,213 bp in size with 81.5% A+T content. It consists of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA), 2 ribosomal RNA (rRNA) genes and an A+T-rich region. All PCGs start with ATN codon, except for COI, which begins with TTAG. Eleven PCGs stop with typical stop codon TAA and TAG. But ND6 and COII use incomplete stop codon T. All of the 22 tRNAs have the typical cloverleaf structure except for tRNASer(AGN) lacking of the dihydrouridine (DHU) stem. The conserved motif ATACTA, poly-T stretch, ATAGA, ATTTA and microsatellite-like (AT)11 were found in the intergenic region between tRNASer(UCN) and ND1 and the A+T-rich region of L. haraldusalis, respectively.
我们已经测定了1859年沃克所描述的哈氏丽夜蛾（Lista haraldusalis）的完整线粒体基因组。哈氏丽夜蛾的线粒体基因组大小为15213碱基对，A+T含量为81.5%。它由13个蛋白质编码基因（PCGs）、22个转运RNA（tRNA）、2个核糖体RNA（rRNA）基因和一个富含A+T的区域组成。除细胞色素氧化酶亚基I（COI）以TTAG起始外，所有PCGs均以ATN密码子起始。11个PCGs以典型的终止密码子TAA和TAG终止。但ND6和细胞色素氧化酶亚基II（COII）使用不完全终止密码子T。22个tRNA中，除了缺乏二氢尿嘧啶（DHU）茎的丝氨酸tRNA（AGN）外，其余均具有典型的三叶草结构。在哈氏丽夜蛾的丝氨酸tRNA（UCN）和ND1之间的基因间隔区以及富含A+T的区域分别发现了保守基序ATACTA、多聚T序列、ATAGA、ATTTA和微卫星样序列（AT）11 。
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