Institute of Plant Protection, Shanxi Academy of Agriculture Science, Taiyuan 030032, China.
Gene. 2013 Sep 10;526(2):277-83. doi: 10.1016/j.gene.2013.05.036. Epub 2013 Jun 3.
Sasakia funebris, a member of the lepidopteran family, Nymphalidae (superfamily Papilionoidea) is a rare species and is found only in some areas of South China. In this study, the 15,233 bp long complete mitochondrial genome of S. funebris was determined, and harbors the gene arrangement identical to all other sequenced lepidopteran insects. The nucleotide composition of the genome is highly A+T biased, accounting for 81.2%. All protein-coding genes (PCGs) start with typical ATN codons, except for COI which begins with the CGA codon. All tRNAs have a typical clover-leaf secondary structure, except for tRNASer(AGN), the dihydrouridine (DHU) arm of which forms a simple loop. The S. funebris A+T-rich region of 370 bp contains several features common to the Lepidoptera insects, including the motif ATAGA followed by a 19 bp poly-T stretch, and two tandem repeats consisting of 18 bp repeat units and 14 bp repeat units. The phylogenetic analyses of Apaturinae based on mitogenome sequences showed: (S. funebris+Sasakia charonda)+(Apatura metis+Apatura ilia). This result is consistent with the morphological classification.
裳凤蝶和曙凤蝶，属于鳞翅目凤蝶科（超科：凤蝶总科），是一种珍稀物种，仅在中国南方的一些地区发现。本研究测定了裳凤蝶的完整线粒体基因组，长度为 15233bp，基因排列与所有已测序鳞翅目昆虫相同。基因组的核苷酸组成高度偏向 A+T，占 81.2%。所有蛋白编码基因（PCGs）均以典型的 ATN 密码子开始，除了 COI 以 CGA 密码子开始。所有 tRNA 都具有典型的三叶草二级结构，除了 tRNASer(AGN)，其 DHU 臂形成简单的环。S. funebris 的 A+T 富含区 370bp 包含几个与鳞翅目昆虫共同的特征，包括 ATAGA -motif 后面跟着 19bp 的多 T 延伸，以及由 18bp 重复单元和 14bp 重复单元组成的两个串联重复。基于线粒体基因组序列的 Apaturinae 系统发育分析显示：（裳凤蝶+曙凤蝶）+（金裳凤蝶+绿带翠凤蝶）。这一结果与形态分类学一致。
