Chime Salome Amarachi, Onyishi Ikechukwu V, Ugwoke Paschal U, Attama Anthony A
1Department of Pharmaceutical Technology and Industrial Pharmacy, University of Nigeria, Nsukka, Nigeria.
J Diet Suppl. 2014 Mar;11(1):7-18. doi: 10.3109/19390211.2013.859212. Epub 2014 Jan 10.
Gongronema latifolium Benth Hook, (Asclepiadaceae) is a tropical rainforest plant primarily used in traditional folk medicine in the treatment of malaria, diabetes, hypertension, and as laxative.
To study the antidiabetic properties of Gongronema latifolium-loaded solid lipid microparticles (SLMs).
The methanolic extract of Gongronema latifolium leaves were obtained and formulated into SLMs using lipid matrix comprising of fat from Capra hircus and Phospholipon® 90H. The SLMs were characterized in vitro by determining the particle size and morphology, pH stability studies, and encapsulation efficiency EE%. Also, the antidiabetic properties of the Gongronema latifolium-loaded SLMs were studied in alloxan-induced diabetic rats.
The results show that the particle size of G. latifolium-loaded SLMs was within an acceptable range for SLMs. Highest EE% of 68% was obtained for SLMs containing 5% G. latifolium (batch F3). The formulations remained stable with insignificant pH reduction over time (p < 0.05). Gongronema latifolium-loaded SLMs had mean percentage reduction in blood glucose of 76% at 2 h, 42.3% at 8 h, and 24.4% at 12 h, while the group that received the reference-glibenclamide had 82.6, 61.7, and 46.7% at 2, 8, and 12 h, respectively, after oral administration of all samples. Gongronema latifolium-loaded SLMs had blood glucose reduction significantly higher than the pure extract and the glibenclamide (p < 0.05) at the concentrations used.
Gongronema latifolium-loaded SLMs exhibited a good hypoglycemic effect and could be used for the treatment of diabetes.
宽叶吊灯花（萝藦科）是一种热带雨林植物，主要用于传统民间医学治疗疟疾、糖尿病、高血压，并用作泻药。
研究载宽叶吊灯花固体脂质微粒（SLMs）的抗糖尿病特性。
获得宽叶吊灯花叶的甲醇提取物，并使用由山羊脂肪和磷脂90H组成的脂质基质将其制成SLMs。通过测定粒径和形态、pH稳定性研究以及包封率EE%对SLMs进行体外表征。此外，在四氧嘧啶诱导的糖尿病大鼠中研究载宽叶吊灯花SLMs的抗糖尿病特性。
结果表明，载宽叶吊灯花SLMs的粒径在SLMs的可接受范围内。含5%宽叶吊灯花的SLMs（批次F3）获得了最高68%的包封率。随着时间推移，制剂保持稳定，pH降低不显著（p<0.05）。口服所有样品后，载宽叶吊灯花SLMs在2小时时血糖平均降低百分比为76%，8小时时为42.3%，12小时时为24.4%，而接受参比药物格列本脲的组在2、8和12小时时分别为82.6%、61.7%和46.7%。在所使用的浓度下，载宽叶吊灯花SLMs的血糖降低显著高于纯提取物和格列本脲（p<0.05）。
载宽叶吊灯花SLMs表现出良好的降血糖作用，可用于治疗糖尿病。
