Bassan Paul, Mellor Joe, Shapiro Jonathan, Williams Kaye J, Lisanti Michael P, Gardner Peter
Manchester Institute of Biotechnology, University of Manchester , 131 Princess Street, Manchester M1 7DN, U.K.
Anal Chem. 2014 Feb 4;86(3):1648-53. doi: 10.1021/ac403412n. Epub 2014 Jan 17.
Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C-H, N-H, and O-H stretches occurring at ca. 2500-3800 cm(-1). The "fingerprint" region of the IR spectrum occurring below 1800 cm(-1) is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found.
傅里叶变换红外（FT-IR）透射模式化学成像传统上是在昂贵的中红外透明窗口（如氟化钡/钙）上进行的，这些窗口比玻璃更易碎，使得在组织病理学实验室中的制备更加繁琐。本文提出了一种解决方案，即使用廉价的玻璃基板进行FT-IR化学成像，该基板具有高波数透射窗口，可测量约2500-3800 cm(-1)处出现的C-H、N-H和O-H伸缩振动。红外光谱低于1800 cm(-1)的“指纹”区域无法获得；然而，我们以71例乳腺组织微阵列（TMA）患者为研究模型，证明在高波数范围内包含了丰富的信息。重要的是，我们证明组织可以分为四种基本组织细胞类型，并且仅使用上皮细胞，就可以对正常组织和恶性组织进行合理区分。
