Xiao J, Zhai H, Yao Y, Wang C, Jiang W, Zhang C, Simard A R, Zhang R, Hao J
Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Département de Chimie et Biochimie, Université de Moncton, Moncton, New Brunswick E1A 3E9, Canada.
Neuroscience. 2014 Mar 14;262:156-64. doi: 10.1016/j.neuroscience.2014.01.004. Epub 2014 Jan 10.
Guillain-Barré syndrome (GBS) is an acute, post-infectious, immune-mediated, demyelinating disease of peripheral nerves and nerve roots. Experimental autoimmune neuritis (EAN) is an animal model of GBS. Chrysin, which is a naturally occurring flavonoid, exhibits various biological activities. This study was designed to investigate the anti-inflammatory and neuroprotective properties of preventative and therapeutic chrysin treatment in EAN rats. For preventative treatment, chrysin was administered orally from day 1 to day 16 (50mg/kg once daily) while, for therapeutic treatment, rats received chrysin from day 7 to day 16 at the same dose once daily. Control animals received the same volume of the vehicle (phosphate-buffered saline/2% dimethylsulfoxide). Regardless of the treatment regimen, chrysin attenuated the severity and duration of the clinical course of EAN and reduced inflammatory cell infiltration and demyelination of sciatic nerves. In the sciatic nerves, the expression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear factor kappa B was reduced. Furthermore, chrysin inhibited the splenic mononuclear cell secretion of interleukin-1β, interleukin-2, interleukin-6, inteleukin-12, interferon γ and tumor necrosis factor α, and elevated the level of inteleukin-4. In summary, our data demonstrate that chrysin is a potentially useful agent for the treatment of EAN with its anti-inflammatory and neuroprotective effects.
吉兰 - 巴雷综合征（GBS）是一种急性、感染后、免疫介导的周围神经和神经根脱髓鞘疾病。实验性自身免疫性神经炎（EAN）是GBS的动物模型。白杨素是一种天然存在的黄酮类化合物，具有多种生物学活性。本研究旨在探讨预防性和治疗性给予白杨素对EAN大鼠的抗炎和神经保护特性。对于预防性治疗，从第1天至第16天口服白杨素（50mg/kg，每日一次），而对于治疗性治疗，大鼠从第7天至第16天接受相同剂量的白杨素，每日一次。对照动物接受相同体积的赋形剂（磷酸盐缓冲盐水/2%二甲基亚砜）。无论治疗方案如何，白杨素均可减轻EAN临床病程的严重程度和持续时间，并减少坐骨神经的炎性细胞浸润和脱髓鞘。在坐骨神经中，诱导型一氧化氮合酶、环氧化酶 - 2和核因子κB的表达降低。此外，白杨素抑制脾单核细胞分泌白细胞介素 - 1β、白细胞介素 - 2、白细胞介素 - 6、白细胞介素 - 12、干扰素γ和肿瘤坏死因子α，并提高白细胞介素 - 4的水平。总之，我们的数据表明，白杨素凭借其抗炎和神经保护作用，是一种治疗EAN的潜在有用药物。
