AFEX 和 H-AFEX 处理的玉米秸秆组成变化对酶解的影响。
Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
机构信息
College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu 210031, China; National Engineering Research Center for Wood-based Resource Utilization, School of Engineering, Zhejiang A&F University, Linan, Zhejiang 311300, China.
College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu 210031, China.
出版信息
Bioresour Technol. 2014 Mar;155:34-40. doi: 10.1016/j.biortech.2013.12.091. Epub 2013 Dec 28.
Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock. This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide. The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated. Results showed that most of the polysaccharides remained intact following each of these two methods. Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose. The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.% hydrogen peroxide loading, and 130°C for 10min. The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
玉米秸秆是被认为可用于生产纤维素乙醇的主要农业废弃物之一。本研究评估了 AFEXTM(1)预处理对玉米秸秆转化为可发酵糖的效果，包括有无在过氧 化氢中预浸泡。对 AFEX 和 H-AFEX 处理后的生物质的组成变化和酶解性能进行了研究。结果表明，这两种方法都能使大多数多糖保持完整。与 AFEX 预处理相比，H-AFEX 工艺提高了木质素的脱除率和葡萄糖及木糖的酶水解产率。H-AFEX 工艺的最大葡聚糖和木聚糖酶解率分别为 87.78%和 90.64%，在 0.7（w/w）水载量、1.0（w/w）氨载量、0.5（w/w）30wt.%过氧化氢载量和 130°C 下处理 10min 时获得。本研究结果表明，H-AFEX 是一种可行的预处理方法，可提高玉米秸秆的酶解糖化性能，用于生物乙醇生产。
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