Bals Bryan D, Gunawan Christa, Moore Janette, Teymouri Farzaneh, Dale Bruce E
MBI, 3815 Technology Boulevard, Lansing, Michigan, 48910-8596.
Biotechnol Bioeng. 2014 Feb;111(2):264-71. doi: 10.1002/bit.25022. Epub 2013 Sep 20.
Ammonia fiber expansion (AFEX™) pretreatment can be performed at small depots, and the pretreated biomass can then be pelletized and shipped to a centralized refinery. To determine the feasibility of this approach, pelletized AFEX-treated corn stover was hydrolyzed at high (18-36%) solid loadings. Water absorption and retention by the pellets was low compared to unpelletized stover, which allowed enzymatic hydrolysis slurries to remain well mixed without the need for fed-batch addition. Glucose yields of 68% and xylose yields of 65% were obtained with 20 mg enzyme/g glucan and 18% solid loading after 72 h, compared to 61% and 59% for unpelletized corn stover. Pelletization also slightly increased the initial rate of hydrolysis compared to unpelletized biomass. The ease of mixing and high yields obtained suggests that pelletization after AFEX pretreatment could have additional advantages beyond improved logistical handling of biomass.
氨纤维膨胀（AFEX™）预处理可在小型仓库进行，预处理后的生物质随后可制成颗粒并运往集中精炼厂。为确定该方法的可行性，对AFEX处理后的玉米秸秆颗粒在高（18 - 36%）固体负荷下进行水解。与未制粒的秸秆相比，颗粒的吸水率和持水率较低，这使得酶解浆液无需分批补料即可保持良好混合。在72小时后，每克葡聚糖使用20毫克酶和18%的固体负荷，葡萄糖产率达到68%，木糖产率达到65%，相比之下，未制粒的玉米秸秆葡萄糖产率和木糖产率分别为61%和59%。与未制粒的生物质相比，制粒还略微提高了水解初始速率。易于混合和高产率表明，AFEX预处理后制粒除了改善生物质的物流处理外，可能还有其他优势。
