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通过联合水力空化和酶预处理实现玉米芯脱木质素:采用响应面法进行工艺优化

Delignification of corncob via combined hydrodynamic cavitation and enzymatic pretreatment: process optimization by response surface methodology.

作者信息

Thangavelu Kiruthika, Desikan Ramesh, Taran Oxana P, Uthandi Sivakumar

机构信息

1Department of Bioenergy, Agricultural Engineering College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003 India.

2Department of Chemistry, Boreskov Institute of Catalysis, Novosibirsk, 630090 Russia.

出版信息

Biotechnol Biofuels. 2018 Jul 24;11:203. doi: 10.1186/s13068-018-1204-y. eCollection 2018.

DOI:10.1186/s13068-018-1204-y
PMID:30061925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6057035/
Abstract

BACKGROUND

Renewable liquid biofuel production will reduce crude oil import of India. To displace the huge quantity of fossil fuels used for energy production, this research was focused on utilization of unexploited low-cost agricultural residues for biofuel production. Corncobs are a byproduct of corn processing industry, and till now it is not utilized for biofuel production, eventhough it has high lignocellulosic concent. In this study, combined hydrodynamic cavitation and enzymatic (HCE) method was evaluated as a pretreatment method of corncob for biofuel production. The most significant process parameters namely (i) enzyme loading (3-10 U g), (ii) biomass loading (2.5-5.0%), and (iii) duration (5-60 min) were optimized and their effects on combined HCE pretreatment of corncob was studied through response surface methodology for lignin reduction, hemicellulose reduction and cellulose increase.

RESULTS

The highest lignin reduction (47.4%) was obtained in orifice plate 1 (OP) under the optimized conditions namely biomass loading at 5%, enzyme loading at 6.5 U g of biomass, and reaction duration of 60 min. The above tested independent variables had a significant effect on lignin reduction. The cavitational yield and energy consumption under the above-mentioned optimized conditions for OP was 3.56 × 10 g J and 1.35 MJ kg, respectively.

CONCLUSIONS

It is evident from the study that HCE is an effective technology and requires less energy (1.35 MJ kg) than other biomass pretreatment methods.

摘要

背景

可再生液体生物燃料的生产将减少印度的原油进口。为了替代大量用于能源生产的化石燃料,本研究聚焦于利用未开发的低成本农业残余物来生产生物燃料。玉米芯是玉米加工业的副产品,尽管其木质纤维素含量高,但迄今为止尚未用于生物燃料生产。在本研究中,评估了联合水力空化和酶解(HCE)方法作为玉米芯生物燃料生产预处理方法的效果。通过响应面法对木质素减少、半纤维素减少和纤维素增加进行研究,优化了最显著的工艺参数,即(i)酶负载量(3 - 10 U g)、(ii)生物质负载量(2.5 - 5.0%)和(iii)持续时间(5 - 60分钟),并研究了它们对玉米芯联合HCE预处理的影响。

结果

在优化条件下,即生物质负载量为5%、酶负载量为每克生物质6.5 U、反应持续时间为60分钟时,在孔板1(OP)中获得了最高的木质素减少率(47.4%)。上述测试的自变量对木质素减少有显著影响。在上述OP优化条件下,空化产率和能量消耗分别为3.56×10 g J和1.35 MJ kg。

结论

该研究表明,HCE是一种有效的技术,与其他生物质预处理方法相比,所需能量更少(1.35 MJ kg)。

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