Department of Biology, Princeton University, 08540, Princeton, NJ, USA.
Planta. 1978 Jan;138(3):295-7. doi: 10.1007/BF00386824.
A rapid method is described of obtaining callus tissue cultures from hypocotyls of vegetative and flowering Xanthium strumarium L. seedlings. The tissue is grown on Murashige and Skoog medium modified with 1 g/l casein hydrolysate and 5 mg/l each of kinetin and α-napthaleneacetic acid.
现介绍一种从拟南芥营养和生殖幼苗下胚轴获得愈伤组织培养物的快速方法。该组织在添加 1g/L 水解酪蛋白和 5mg/L 激动素和α-萘乙酸的改良 Murashige 和 Skoog 培养基上生长。