Department of Biological Science, Rani Durgavati University, M.P.-482 001, Jabalpur, India.
World J Microbiol Biotechnol. 1995 Mar;11(2):193-5. doi: 10.1007/BF00704647.
Protoplasts of a catabolite-repression-resistant strain ofMalbranchea sulfurea and a mutant of it over-producing amylase were isolated and fused using electrofusion. The yield of hybrids was 5×10(-5). One stable hybrid, DGCS 1, was insensitive to glucose repression and produced approx. twice the α-amylase activity produced by either of its parents. The amount of DNA in DGCS 1 was also double that of either parent strain.
用原生质体电融合的方法，分别对分解代谢阻遏物抗性的硫丝菌属和产淀粉酶突变株进行了原生质体融合。杂种的产量为 5×10(-5)。一个稳定的杂种 DGCS 1 对葡萄糖阻遏不敏感，产生的 α-淀粉酶活力比其亲本大约高二倍。DGCS 1 的 DNA 含量也比亲本菌株的两倍还多。
