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短讯：利用热稳定几丁质酶和枝顶孢霉的昆布多糖酶从嗜热真菌硫色多孔菌中形成原生质体。

Short communication: Protoplast formation from the thermophilic fungus Malbranchea sulfurea, using the thermostable chitinase and laminarinase of Paecilomyces varioti.

机构信息

Department of Biological Science, Rani Durgavati University, MP - 482 001, Jabalpur, India.

出版信息

World J Microbiol Biotechnol. 1996 Jan;12(1):99-100. doi: 10.1007/BF00327811.

DOI:10.1007/BF00327811
PMID:24415099
Abstract

Two thermostable enzymes produced by the thermophilic fungus Paecilomyces varioti, a chitinase and laminarinase, were used to isolate protoplasts of a thermophilic fungus, Malbranchea sulfurea. The frequency of protoplast regeneration observed (35%) was considerably higher than that obtained using commercial lytic enzymes.

摘要

两株嗜热真菌——佩氏拟青霉所产生的热稳定性酶，即几丁质酶和昆布多糖酶，被用于从嗜热真菌硫色多孔菌中分离原生质体。观察到的原生质体再生频率（35%）明显高于使用商业裂解酶获得的再生频率。

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