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减数分裂中的核型分析:黑麦属(Secale L.)的吉姆萨带型

Karyotype analysis in meiosis: Giemsa banding in the genus Secale L.

机构信息

Fachbereich Biologie (Botanik), J.W. Goethe-Universität, Frankfurt a. M., BRD.

出版信息

Theor Appl Genet. 1976 May;47(3):101-7. doi: 10.1007/BF00274937.

DOI:10.1007/BF00274937
PMID:24414528
Abstract

Terminal bands of meiotic chromosomes stained by the Giemsa technique are permanent genetic structures of the nucleus during PMC differentiation in 8 samples of wild, primitive, and cultivated species of rye. The characteristic meiotic banding pattern is probably identical with the heterochromatic regions of mitotic chromosomes of root meristem cells (RMC) which have so far been studied. Karyotype analysis can be significantly improved by quantitative studies of the number and size of the bands combined with certain well-known chromosome characters in diplotene and diakinesis. The chromosomes involved in multivalents of some natural and synthetic species hybrids are identified for the first time. The results are discussed both in relation to the problems of chromosome evolution and their significance for marker techniques in cytogenetics.

摘要

在 8 个野生、原始和栽培黑麦品种的 PMC 分化过程中,通过吉姆萨技术染色的减数分裂染色体端带是核的永久性遗传结构。这种有特点的减数分裂带型可能与迄今为止研究过的根分生组织细胞(RMC)有丝分裂染色体的异染色质区域相同。通过结合二分体和减数分裂前期二的某些已知染色体特征对带的数量和大小进行定量研究,可以显著改善染色体组型分析。首次鉴定了一些自然和合成种杂种的多价体所涉及的染色体。结果与染色体进化问题及其在细胞遗传学标记技术中的意义有关进行了讨论。

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引用本文的文献

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Nat Commun. 2023 May 18;14(1):2846. doi: 10.1038/s41467-023-38543-0.
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The DNA content of the individual chromosomes of rye.黑麦个体染色体的 DNA 含量。
Theor Appl Genet. 1982 Jun;62(2):145-8. doi: 10.1007/BF00293347.

本文引用的文献

1
Chromomere analysis of the chromosome complement of rye.黑麦染色体组的染色粒分析
Chromosoma. 1952;5(1):1-68. doi: 10.1007/BF01271482.
2
An improved Giemsa C-banding procedure for plant chromosomes.一种改良的植物染色体吉姆萨C带技术。
Experientia. 1974 May 15;30(5):570-1. doi: 10.1007/BF01926356.