Robert Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, S10 2TN, Sheffield, UK.
Photosynth Res. 1991 Feb;27(2):121-33. doi: 10.1007/BF00033251.
Non-photochemical chlorophyll fluorescence quenching (qN) in barley leaves has been analysed by monitoring its relaxation in the dark, by applying saturating pulses of light. At least three kinetically distinct phases to qN recovery are observed, which have previously been identified (Quick and Stitt 1989) as being due to high-energy state quenching ('fast'), excitation energy redistribution due to a state transition ('medium') and photoinhibition ('slow'). However, measurements of chlorophyll fluorescence at 77 K from leaf extracts show that state transitions only occur in low light conditions, whereas the 'medium' component of qN is very large in high light. The source of that part of the 'medium' component not accounted for by a state transition is discussed.
已通过暗弛豫监测和施加饱和光脉冲的方式分析大麦叶片非光化学叶绿素荧光猝灭（qN）。观察到 qN 恢复至少存在三个动力学不同的相，此前已确定（Quick 和 Stitt，1989）归因于高能态猝灭（“快”）、由于态转变引起的激发能再分配（“中”）和光抑制（“慢”）。然而，从叶提取物中测量 77 K 时的叶绿素荧光表明，仅在低光照条件下发生态转变，而在高光下 qN 的“中”成分非常大。讨论了不能用态转变解释的“中”成分的那部分的来源。
