Botanisches Institut der Universität, Universitätsstr. 1, D-4000, Düsseldorf, F.R.G..
Photosynth Res. 1989 Nov;22(2):137-46. doi: 10.1007/BF00035444.
The effects of the tertiary amines tetracaine, brucine and dibucaine on photophosphorylation and control of photosynthetic electron transport in isolated chloroplasts of Spinacia oleracea were investigated. Tertiary amines inhibited photophosphorylation while the related electron transport decreased to the rates, observed under non-phosphorylating conditions. Light induced quenching of 9-aminoacridine fluorescence and uptake of (14)C-labelled methylamine in the thylakoid lumen declined in parallel with photophosphorylation, indicating a decline of the transthylakoid proton gradient. In the presence of ionophoric uncouplers such as nigericin, no effect of tertiary amines on electron transport was seen in a range of concentration where photophosphorylation was inhibited. Under the influence of the tertiary amines tested, pH-dependent feed-back control of photosystem II, as indicated by energy-dependent quenching of chlorophyll fluorescence, was unaffected or even increased in a range of concentration where 9-aminoacridine fluorescence quenching and photophosphorylation were inhibited. The data are discussed with respect to a possible involvement of localized proton flow pathways in energy coupling and feed-back control of electron transport.
研究了叔胺（包括丁卡因、苯佐卡因和士的宁）对菠菜叶绿体离体光合作用磷酸化和电子传递的影响。叔胺抑制磷酸化作用，而相关的电子传递则下降到非磷酸化条件下观察到的速率。9-氨基吖啶荧光的光诱导猝灭和类囊体腔中 14C 标记甲胺的摄取与磷酸化平行下降，表明跨类囊体质子梯度下降。在离子载体解偶联剂（如尼可刹米）存在下，在磷酸化受到抑制的浓度范围内，叔胺对电子传递没有影响。在测试的叔胺的影响下，由叶绿素荧光能量依赖性猝灭指示的光系统 II 的 pH 依赖性反馈控制不受影响，甚至在 9-氨基吖啶荧光猝灭和磷酸化受到抑制的浓度范围内增加。这些数据与局部质子流途径在能量偶联和电子传递反馈控制中的可能参与有关。
