Robert Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, S10 2TN, Sheffield, UK.
Photosynth Res. 1990 May;24(2):167-73. doi: 10.1007/BF00032596.
The relaxation of the non-photochemical quenching of chlorophyll fluorescence has been investigated in cells of the green alga Dunaliella following illumination. The relaxation after the addition of DCMU or darkening was strongly biphasic. The uncoupler NH4Cl induced rapid relaxation of both phases, which were therefore both energy-dependent quenching, qE. The proportion of the slow phase of qE increased at increasing light intensity. In the presence of the inhibitors rotenone and antimycin the slow phase of qE was stabilised for in excess of 15 min. NaN3 inhibited the relaxation of almost all the qE. The implications of these results are discussed in terms of the interpretation of the non-photochemical quenching of chlorophyll fluorescence in vivo and the mechanism of qE.
已研究了在照光后绿藻杜氏盐藻细胞中叶绿素荧光非光化学猝灭的弛豫。加入 DCMU 或变暗后弛豫呈强烈的双相性。解偶联剂 NH4Cl 诱导两个相的快速弛豫，因此两个相都是能量依赖的猝灭，qE。qE 的慢相比例随光强的增加而增加。在抑制剂鱼藤酮和抗霉素的存在下，qE 的慢相稳定超过 15 分钟。NaN3 几乎抑制了所有 qE 的弛豫。根据这些结果讨论了在体内解释叶绿素荧光非光化学猝灭和 qE 机制的意义。
