Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, 94305, Stanford, CA, USA.
Photosynth Res. 1991 Feb;27(2):151-6. doi: 10.1007/BF00033254.
A photoacoustic cell assembly is described that is permeable to CO2 and other gases but not water vapor. As a replacement for the usually employed solid cover, this cell uses a cover containing a small fritted glass disk that holds a small piece of 6.4 μm Teflon film against the sample.With the above arrangement it was possible to increase the rate of O2 evolution measured photoacoustically about 3 times in Zea mays leaves and about 1.7 times in Phaseolus vulgaris leaves upon adding CO2 to the gas stream. The extent of energy storage was also enhanced with supplemental CO2 in Zea and Ulva but less so in Phaseolus. The maximum improvements of photosynthetic activities were obtained when the gas stream contained 2.5-5% CO2. These high concentrations were presumably necessary as the result of a high resistance to diffusion through the gas-permeable cover.
描述了一种可渗透 CO2 和其他气体但不能渗透水蒸气的光声细胞组件。作为通常使用的固体盖的替代品,该细胞使用包含小的烧结玻璃盘的盖,该小的烧结玻璃盘将一小片 6.4μm 的聚四氟乙烯薄膜固定在样品上。通过这种布置,可以将玉米叶片中光声测量的 O2 释放速率提高约 3 倍,在添加 CO2 到气流中时,菜豆叶片中的释放速率提高约 1.7 倍。在 Zea 和 Ulva 中,能量存储的程度也随着补充 CO2 而增强,但在 Phaseolus 中则不然。当气流中含有 2.5-5%的 CO2 时,可获得光合作用活性的最大提高。这些高浓度可能是由于通过透气盖的扩散阻力很高所致。