Nevins D J, English P D, Albersheim P
Department of Chemistry, University of Colorado, Boulder, Colorado 80302.
Plant Physiol. 1967 Jul;42(7):900-6. doi: 10.1104/pp.42.7.900.
Polysaccharide compositions of cell walls were assessed by quantitative analyses of the component sugars. Cell walls were hydrolyzed in 2 n trifluoroacetic acid and the liberated sugars reduced to their respective alditols. The alditols were acetylated and the resulting alditol acetates separated by gas chromatography. Quantitative assay of the alditol acetates was accomplished by electronically integrating the detector output of the gas chromatograph. Myo-inositol, introduced into the sample prior to hydrolysis, served as an internal standard.The cell wall polysaccharide compositions of plant varieties within a given species are essentially identical. However, differences in the sugar composition were observed in cell walls prepared from different species of the same as well as of different genera. The fact that the wall compositions of different varieties of the same species are the same indicates that the biosynthesis of cell wall polysaccharides is genetically regulated. The cell walls of various morphological parts (roots, hypocotyls, first internodes and primary leaves) of bean plants were each found to have a characteristic sugar composition.It was found that the cell wall sugar composition of suspension-cultured sycamore cells could be altered by growing the cells on different carbon sources. This demonstrates that the biosynthesis of cell wall polysaccharides can be manipulated without fatal consequences.
通过对细胞壁组成糖类进行定量分析来评估细胞壁的多糖成分。细胞壁在2N三氟乙酸中水解，释放出的糖还原为各自的糖醇。糖醇进行乙酰化，所得的糖醇乙酸酯通过气相色谱法分离。通过对气相色谱仪检测器输出进行电子积分来完成对糖醇乙酸酯的定量测定。在水解前引入样品中的肌醇用作内标。同一物种内不同植物品种的细胞壁多糖成分基本相同。然而，在同一属不同种以及不同属不同种制备的细胞壁中观察到了糖成分的差异。同一物种不同品种的细胞壁成分相同这一事实表明细胞壁多糖的生物合成受基因调控。发现豆类植物不同形态部位（根、下胚轴、第一节间和初生叶）的细胞壁各自具有独特的糖成分。研究发现，通过在不同碳源上培养悬浮培养的悬铃木细胞，其细胞壁糖成分会发生改变。这表明细胞壁多糖的生物合成可以被调控而不会产生致命后果。
