Department of Plant Physiology, Eötvös University, P.O. Box 324, H-1445, Budapest 8, Hungary.
Planta. 1978 Jan;141(2):135-9. doi: 10.1007/BF00387879.
Light-induced fluorescence decay was examined during the greening of control and lincomycintreated maize (Zea mays L.) leaves. Assuming that this decay to a first approximation is the result of two parallel first-order reactions, the fluorescence induction curves were linearized on the logarithm plot and the parameters were determined. The variable fluorescence increased, and the parameters of the two linear sections of the fluorescence decay-that is, the kinetics of the induction curves-changed during the greening of the control leaves. Lincomycin treatment caused some chlorophyll deficiency and the lowering of the chlorophyll a/b ratio, changed the fluorescence emission spectra and the effect of Mg(2+) on the regulation of the excitation energy distribution. The structure of the thylakoids and the kinetics of the fluorescence decay were also changed in the treated leaves. The possible relationship between the change of the kinetics of the fluorescence decay and the change of spillover during greening and after lincomycin treatment is discussed.
光照诱导荧光衰减在对照和林可霉素处理的玉米(Zea mays L.)叶片的变绿过程中进行了研究。假设这种衰减在第一近似下是两个平行的一级反应的结果,荧光诱导曲线在对数图上线性化,并确定了参数。在对照叶片变绿过程中,可变荧光增加,荧光衰减的两个线性部分的参数-即诱导曲线的动力学-发生了变化。林可霉素处理导致一些叶绿素缺失和叶绿素 a/b 比值降低,改变了荧光发射光谱以及 Mg(2+)对激发能分布的调节作用。处理叶片中的类囊体结构和荧光衰减动力学也发生了变化。讨论了变绿过程中和林可霉素处理后荧光衰减动力学变化与溢出现象变化之间的可能关系。