Department of Biology, University of California, 92502, Riverside, CA, USA.
Planta. 1978 Jan;141(3):269-72. doi: 10.1007/BF00388342.
The effects of 5-bromo-2-deoxyuridine (BUdR, thymidine analogue), AMO-1618 (2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride), a growth retardant, and p-chlorophenoxyisobutyric acid (PCIB, an antiauxin) on growth (dry weight increase) and fiber development in unfertilized cotton (Gossypium hirsutum L.) ovules grown in vitro have been studied. BUdR (5 μM) causes about 70% inhibition of fiber production, with little effect on ovule growth, if applied during the first 6 d of culture in the presence of GA3 and IAA. AMO-1618, when used with GA3 alone, causes only a small reduction in both dry weight and fiber production, but when used with IAA alone reduces both fiber production and dry weight, the effect on the latter being predominant. In the presence of both IAA and GA3, AMO-1618 causes a small decrease in fiber production but a major decrease in dry weight. PCIB completely inhibits fiber growth but has little effect on dry weight, especially when GA3 is present. These results indicate that GA3 mainly promotes ovule growth while IAA is largerly responsible for fiber growth.
研究了 5-溴-2-脱氧尿苷（BUdR，胸苷类似物）、AMO-1618（2-异丙基-4-二甲基氨基-5-甲基苯基-1-哌啶羧酸甲酯盐酸盐）、生长抑制剂和对氯苯氧异丁酸（PCIB，一种抗生长素）对体外培养的未受精棉花（Gossypium hirsutum L.）胚珠生长（干重增加）和纤维发育的影响。如果在 GA3 和 IAA 存在的情况下，在培养的前 6 天内应用，BUdR（5 μM）会导致纤维产生约 70%的抑制，对胚珠生长几乎没有影响。单独使用 AMO-1618 时，对干重和纤维产生的减少都很小，但单独使用 IAA 时，纤维产生和干重都会减少，后者的影响更为明显。在 IAA 和 GA3 共存的情况下，AMO-1618 会导致纤维产生量略有减少，但干重明显减少。PCIB 完全抑制纤维生长，但对干重影响很小，尤其是在存在 GA3 的情况下。这些结果表明，GA3 主要促进胚珠生长，而 IAA 主要负责纤维生长。
