Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing, P.R. China.
Nat Biotechnol. 2011 May;29(5):453-8. doi: 10.1038/nbt.1843. Epub 2011 Apr 10.
The capacity of conventional breeding to simultaneously improve the yield and quality of cotton fiber is limited. The accumulation of the plant hormone indole-3-acetic acid (IAA) in cotton fiber initials prompted us to investigate the effects of genetically engineering increased IAA levels in the ovule epidermis. Targeted expression of the IAA biosynthetic gene iaaM, driven by the promoter of the petunia MADS box gene Floral Binding protein 7 (FBP7), increased IAA levels in the epidermis of cotton ovules at the fiber initiation stage. This substantially increased the number of lint fibers, an effect that was confirmed in a 4-year field trial. The lint percentage of the transgenic cotton, an important component of fiber yield, was consistently higher in our transgenic plants than in nontransgenic controls, resulting in a >15% increase in lint yield. Fiber fineness was also notably improved.
常规育种同时提高棉花纤维产量和品质的能力有限。棉花纤维起始细胞中植物激素吲哚-3-乙酸（IAA）的积累促使我们研究在珠被表皮中遗传工程增加 IAA 水平的效果。iaaM 基因的 IAA 生物合成基因的靶向表达，由矮牵牛 MADS 盒基因 Floral Binding protein 7（FBP7）的启动子驱动，在纤维起始阶段增加了棉花胚珠表皮中的 IAA 水平。这显著增加了长绒纤维的数量，这一效应在 4 年的田间试验中得到了证实。在我们的转基因植物中，转基因棉花的纤维百分率（纤维产量的重要组成部分）始终高于非转基因对照，导致纤维产量增加了 15%以上。纤维细度也显著提高。
