Laboratorio de Microbiología, Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P, Universidad Católica de Chile, Alamede 340, Casilla 114-D, Santiago, Chile.
World J Microbiol Biotechnol. 1995 Sep;11(5):536-40. doi: 10.1007/BF00286369.
No microorganisms could be isolated from chemostats or from a soil column fed with 4,5-dichloroguaiacol as the only carbon source. If guaiacol was added to chemostats with 4,5-dichloroguaiacol, either soil microbial consortia or guaiacol-degrading bacteria could dechlorinate the 4,5-dichloroguaiacol provided it was <0.2MM. A microbial consortium from farm soil removed 4,5-dichloroguaiacol under aerobic or anoxic conditions, with or without chlorolignin. Dichlorocatechol was the only 4,5-dichloroguaiacol-derived metabolite detected. In aerobic incubations, 4,5-dichlorocatechol was further degraded whereas under anoxic conditions it accumulated.
从恒化器或仅用 4,5-二氯愈创木酚作为唯一碳源的土壤柱中均无法分离到微生物。如果在含有 4,5-二氯愈创木酚的恒化器中添加愈创木酚，土壤微生物群落或愈创木酚降解菌可以将 4,5-二氯愈创木酚脱氯，只要浓度<0.2MM。来自农田土壤的微生物群落可以在有氧或缺氧条件下，有或没有氯木质素的情况下，去除 4,5-二氯愈创木酚。检测到的唯一 4,5-二氯愈创木酚衍生代谢物是二氯邻苯二酚。在有氧孵育中，4,5-二氯邻苯二酚进一步降解，而在缺氧条件下则积累。
