短篇通讯：应用 PCR 和荧光抗体方法检测实验室微宇宙中不可培养的霍乱弧菌 O139
Short communication: Detection of non-culturable Vibrio cholerae O139, by PCR and fluorescent antibody methods, in laboratory microcosms.
机构信息
Environmental Microbiology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), GPO Box 128, 1000, Dhaka, Bangladesh.
出版信息
World J Microbiol Biotechnol. 1995 Sep;11(5):597-8. doi: 10.1007/BF00286382.
PMID:24414922
Abstract
Non-culturable Vibrio cholerae O139 was detected in microcosms by PCR and fluorescent-antibody (FA) techniques. When survival of V. cholerae O139 in microcosms was assessed by viable counting on culture media, the vibrio became non-culturable after 44 days and remained non-culturable for an additional 7 weeks.
摘要
通过 PCR 和荧光抗体（FA）技术在微宇宙中检测到不可培养的霍乱弧菌 O139。当通过在培养基上进行活菌计数来评估微宇宙中霍乱弧菌 O139 的存活情况时，该弧菌在 44 天后变得不可培养，并在另外 7 周内保持不可培养。
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