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用于检测霍乱弧菌霍乱肠毒素操纵子的聚合酶链反应。

Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

作者信息

Shirai H, Nishibuchi M, Ramamurthy T, Bhattacharya S K, Pal S C, Takeda Y

机构信息

Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.

出版信息

J Clin Microbiol. 1991 Nov;29(11):2517-21. doi: 10.1128/jcm.29.11.2517-2521.1991.

DOI:10.1128/jcm.29.11.2517-2521.1991
PMID:1774258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC270365/
Abstract

We report a set of oligonucleotide primers and amplification conditions for the polymerase chain reaction to detect the ctx operon of Vibrio cholerae. The results of this assay on strains of V. cholerae and related organisms were identical with those obtained by the DNA colony hybridization test with the polynucleotide probe. The detection limit of this system was 1 pg of chromosomal DNA or broth culture containing three viable cells. The polymerase chain reaction method could directly detect the ctx operon sequences in rice-water stool samples from patients with cholera.

摘要

我们报告了一组用于聚合酶链反应检测霍乱弧菌ctx操纵子的寡核苷酸引物和扩增条件。对霍乱弧菌菌株及相关微生物进行该检测的结果，与使用多核苷酸探针进行DNA菌落杂交试验所得结果一致。该系统的检测限为1 pg染色体DNA或含有三个活细胞的肉汤培养物。聚合酶链反应方法可直接检测霍乱患者米泔水样粪便样本中的ctx操纵子序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/61491421d706/jcm00047-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/dd71b36b7046/jcm00047-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/a252b389c963/jcm00047-0173-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/61491421d706/jcm00047-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/dd71b36b7046/jcm00047-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/a252b389c963/jcm00047-0173-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e788/270365/61491421d706/jcm00047-0174-a.jpg

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