Biologisches Institut II (Botanik), Universität Freiburg i. Br., Schänzlestrasse 1, 7800, Freiburg, Germany.
Photosynth Res. 1991 Jun;28(3):109-18. doi: 10.1007/BF00054124.
A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-dependent degradation of envelope membranes could be prevented by addition of α-tocopherol prior to monoterpene incubation.Photosynthetic electron transport of photosystem II was completely inhibited by β-pinene, Triton X-100 and linolenic acid. Inhibition could be modulated by addition of α-tocopherol or lecithin (phosphatidylcholine) either before or after inhibition by monoterpenes and detergent.Percentage reconstitution of photosynthetic electron transport inhibited by β-pinene depended on light conditions and incubation time.
从南瓜（Cucurbita pepo (L.) var. Senator）中提取叶绿体制剂。通过连续自由流动电泳实现完整叶绿体的富集。添加单萜、去污剂和游离脂肪酸会显著改变电泳分离模式。在单萜孵育前添加α-生育酚可以防止单萜依赖性 envelope 膜降解。β-蒎烯、Triton X-100 和亚油酸完全抑制光系统 II 的光合电子传递。添加α-生育酚或卵磷脂（磷脂酰胆碱）可以在单萜和去污剂抑制之前或之后调节抑制作用。β-蒎烯抑制的光合电子传递的百分比再构成取决于光照条件和孵育时间。
