Rizk Maha Z, Aly Hanan F, Abo-Elmatty Dina M, Desoky M M, Ibrahim N, Younis Eman A
Department of Therapeutical Chemistry, National Research Center, Cairo, Egypt.
Department of Therapeutical Chemistry, National Research Center, Cairo, Egypt
Toxicol Ind Health. 2016 May;32(5):877-907. doi: 10.1177/0748233713503030. Epub 2014 Jan 10.
This study aims to evaluate two proteins derived from the seeds of the plants Cajanus cajan (Leguminosae) and Caesalpinia gilliesii (Leguminosae) for their abilities to ameliorate the toxic effects of chronic doses of acetoaminphen (APAP) through the determination of certain biochemical parameters including liver marker enzymes: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and total bilirubin. Also, total protein content and hepatic marker enzyme, lactate dehydrogenase were studied. Moreover, liver antioxidants, glutathione (GSH), nitric oxide, and lipid peroxides were determined in this study. Hepatic adenosine triphosphatase (ATPase), adenylate energy charge (ATP, adenosine diphosphate, adenosine monophosphate, and inorganic phosphate), and phosphate potential, serum interleukin-6, tumor necrosis factor-α, and myeloperoxidase were also examined in the present study. On the other hand, histopathological examination of intoxicated and liver treated with both proteins was taken into consideration. The present results show disturbances in all biochemical parameters and hepatic toxicity signs including mild vascular congestion, moderate inflammatory changes with moderate congested sinusoids, moderate nuclear changes (pyknosis), moderate centrilobular necrosis, fatty changes, nuclear pyknosis vascular congestion, and change in fatty centrilobular necrosis liver. Improvement in all biochemical parameters studied was noticed as a result of treatment intoxicated liver with C. gilliesii and C. cajan proteins either paracetamol with or post paracetamol treatment. These results were documented by the amelioration signs in rat's hepatic architecture. Thus, both plant protein extracts can upregulate and counteract the inflammatory process, minimize damage of the liver, delay disease progression, and reduce its complications.
本研究旨在评估源自植物木豆（豆科）和刺云实（豆科）种子的两种蛋白质，通过测定某些生化参数，包括肝脏标记酶：丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶和总胆红素，来评估它们改善慢性剂量对乙酰氨基酚（APAP）毒性作用的能力。此外，还研究了总蛋白含量和肝脏标记酶乳酸脱氢酶。而且，本研究还测定了肝脏抗氧化剂、谷胱甘肽（GSH）、一氧化氮和脂质过氧化物。本研究还检测了肝脏腺苷三磷酸酶（ATPase）、腺苷酸能量电荷（ATP、二磷酸腺苷、一磷酸腺苷和无机磷酸盐）以及磷酸势、血清白细胞介素-6、肿瘤坏死因子-α和髓过氧化物酶。另一方面，还考虑了对用这两种蛋白质处理的中毒和肝脏进行组织病理学检查。目前的结果显示所有生化参数和肝脏毒性体征均出现紊乱，包括轻度血管充血、伴有中度充血窦状隙的中度炎症变化、中度核变化（核固缩）、中度小叶中心坏死、脂肪变性、核固缩血管充血以及小叶中心坏死性脂肪肝的变化。在用刺云实和木豆蛋白质处理中毒肝脏后，无论是在对乙酰氨基酚处理前还是处理后，所研究的所有生化参数均有改善。大鼠肝脏结构的改善迹象证明了这些结果。因此，两种植物蛋白提取物均可上调并对抗炎症过程，使肝脏损伤最小化，延缓疾病进展并减少其并发症。
