Department of Environmental Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar (Nainital)-263145, U.P., India.
World J Microbiol Biotechnol. 1996 Jan;12(1):12-5. doi: 10.1007/BF00327791.
A thermophilic fungus, Humicola grisea var thermoidea, produced in liquid culture two endoxylanases (1,4-β-D-xylan-xylanohydrolase, EC 3.2.1.8) with M r of 95 (Xyl I) and 13 (Xyl II) kDa. PAGE of the crude culture filtrate and of each fraction obtained by gel filtration produced three and one band, respectively. Cross-reaction of the culture filtrate and each fraction with polyclonal antibodies prepared against Xyl II produced two and one precipitin bands, respectively. Hydrolysis of wheat straw and rice husk xylan was maximal using a combination of Xyl I and Xyl II. The products formed after hydrolysis, xylo-oligosaccharides and traces of xylose, indicated an endotype enzyme action and the co-operative activities of the xylanases.
嗜热真菌灰绿曲霉经液体培养可产生两种内切木聚糖酶（1,4-β-D-木聚糖-木聚糖水解酶，EC 3.2.1.8），相对分子质量分别为 95（Xyl I）和 13（Xyl II）kDa。粗培养液和凝胶过滤得到的各部分的 PAGE 分别产生 3 条和 1 条带。培养液和各部分与针对 Xyl II 制备的多克隆抗体的交叉反应分别产生 2 条和 1 条沉淀带。Xyl I 和 Xyl II 的组合对小麦秸秆和稻壳木聚糖的水解作用最大。水解后形成的产物，低聚木糖和痕量木糖，表明该酶为内切型，并且木聚糖酶具有协同活性。
