Thakur I S
Department of Environmental Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, India.
Int Arch Allergy Appl Immunol. 1989;90(2):124-9. doi: 10.1159/000235013.
Prosopis juliflora pollen allergen extract was prepared, and its crude allergen extract was fractionated by Sephadex G-100 gel filtration. Six different fractions were obtained which was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Protein and carbohydrate content of each fraction were estimated. Fraction E (MW 20,000) showed a 25% carbohydrate concentration. The amino acid analysis indicated that this fraction was rich in glutamic acid and alanine. Antigenicity or allergenicity of fractionated allergens were checked by gel diffusion test, rocket immunoelectrophoresis, skin prick test, and radioallergosorbent test. All these test indicate that fraction E consisted mainly of allergenic molecules (MW 20,000) of P. juliflora pollen.
制备了朱缨花粉过敏原提取物，并通过葡聚糖G - 100凝胶过滤对其粗过敏原提取物进行分级分离。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳确认得到了六种不同的级分。对每个级分的蛋白质和碳水化合物含量进行了测定。E级分（分子量20,000）显示出25%的碳水化合物浓度。氨基酸分析表明该级分富含谷氨酸和丙氨酸。通过凝胶扩散试验、火箭免疫电泳、皮肤点刺试验和放射变应原吸附试验检查分级分离的过敏原的抗原性或致敏性。所有这些试验表明E级分主要由朱缨花粉的致敏分子（分子量20,000）组成。
