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昆虫醛类信息素的生物发光测定法的建立:III. 气载信息素分析。

Development of a bioluminescence assay for aldehyde pheromones of insects : III. Analysis of airborne pheromone.

机构信息

Department of Biochemistry, McGill University, 3655 Drummond Street, H3G 1 Y6, Montreal, Quebec.

出版信息

J Chem Ecol. 1982 Jun;8(6):935-45. doi: 10.1007/BF00987660.

DOI:10.1007/BF00987660
PMID:24415249
Abstract

A newly developed bioluminescent assay was used to measure quantitatively the amount of (E)-11-tetradecenal, the major component of the sex pheromone of the spruce budworm, trapped on Porapak Q(®). The bioluminescent response was linearly related to the amount of aldehyde either deposited on the absorbent or trapped from an airstream. However, the recovery of pheromone from Porapak was dependent on whether the air was prefiltered (through Porapak) or taken directly from the atmosphere. Furthermore, pheromone on Porapak was lost with time during the flow of air through the absorbent, indicating that trapping of aldehyde pheromone should be conducted for short periods of time for optimal recoveries. The applicability of the assay system for the rapid and direct measurement of the release rates of aldehyde pheromone lures was demonstrated for pheromone lures used for baiting spruce budworm traps.

摘要

一种新开发的生物发光分析方法被用于定量测量(E)-11-十四碳烯醛的含量,这是云杉芽蛾性信息素的主要成分,它被吸附在 Porapak Q(®)上。生物发光反应与醛在吸附剂上的沉积量或从气流中捕获的量呈线性关系。然而,从 Porapak 回收信息素取决于空气是否经过预过滤(通过 Porapak)还是直接取自大气。此外,随着空气通过吸附剂的流动,信息素会随时间从 Porapak 上流失,这表明为了获得最佳回收率,应在短时间内进行醛类信息素的捕获。该分析系统可用于快速直接测量诱芯中醛类信息素的释放率,这在用于诱捕云杉芽蛾诱捕器的诱芯中得到了证明。

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引用本文的文献

1
Development of a bioluminescence assay for aldehyde pheromones of insects : I. Sensitivity and specificity.昆虫醛类信息素的生物发光检测法的建立:I. 灵敏度和特异性。
J Chem Ecol. 1982 Jun;8(6):911-21. doi: 10.1007/BF00987658.
2
Insect sex pheromones: Determination of half-lives from formulations by collection of emitted vapor.昆虫性信息素:通过收集排放的蒸气从制剂中测定半衰期。
J Chem Ecol. 1983 Nov;9(11):1491-502. doi: 10.1007/BF00988515.

本文引用的文献

1
Development of a bioluminescence assay for aldehyde pheromones of insects : II. Analysis of pheromone glands.昆虫醛类信息素的生物发光测定法的建立:Ⅱ.信息素腺分析。
J Chem Ecol. 1982 Jun;8(6):923-33. doi: 10.1007/BF00987659.
2
Development of a bioluminescence assay for aldehyde pheromones of insects : I. Sensitivity and specificity.昆虫醛类信息素的生物发光检测法的建立:I. 灵敏度和特异性。
J Chem Ecol. 1982 Jun;8(6):911-21. doi: 10.1007/BF00987658.
3
Purification and properties of bacterial luciferases.细菌荧光素酶的纯化及性质
J Biol Chem. 1972 Jan 25;247(2):398-404.