Department of Biochemistry, McGill University, 3655 Drummond Street, H3G 1Y6, Montreal, Quebec, Canada.
J Chem Ecol. 1982 Jun;8(6):911-21. doi: 10.1007/BF00987658.
A rapid, quantitative assay for long-chain aldehydes based on bacterial luminescence was developed. Significant luminescent responses were obtained with both saturated and unsaturated aldehydes of 12-18 carbons. Maximum responses were obtained with the 14- and 16-carbon compounds, including those that are known insect sex pheromones. The bioluminescent response was linearly related to the amount of aldehyde over a 10(4)-10(5) concentration range with as little as 0.1 pmol (∼20 pg) of aldehyde being detected. The bioluminescent assay represents a new quantitative tool for rapidly measuring aldehyde pheromones of insects.
基于细菌发光，开发出一种快速定量检测长链醛的方法。该方法对 12-18 个碳的饱和和不饱和醛均有显著的发光响应。在 14 碳和 16 碳化合物中获得了最大的响应，包括已知的昆虫性信息素。生物发光响应与醛的量在 10(4)-10(5)浓度范围内呈线性关系，检测到的醛的量低至 0.1 pmol（约 20 pg）。生物发光测定法为快速测量昆虫的醛类信息素提供了一种新的定量工具。
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