Simons R, Alon N, Riordan J R
Biochem Biophys Res Commun. 1987 Jul 31;146(2):666-71. doi: 10.1016/0006-291x(87)90580-8.
Using a myelin proteolipid protein (PLP) cDNA as probe, a 1kb cDNA was isolated from a human retinal cDNA library. This clone, designated pDM-20, contained all of the coding sequence of PLP except for 105 base pairs which encode amino acids 117-151 of PLP. This deletion would result in a protein of the size of DM-20, the second major proteolipid of Central Nervous System (CNS) myelin. Homology between the DNA sequence at the 5' end of the deletion and the donor splicing consensus sequence suggests that PLP and DM-20 transcripts are derived from a single PLP gene by alternate splicing.
以髓磷脂蛋白脂蛋白（PLP）cDNA为探针，从人视网膜cDNA文库中分离出一个1kb的cDNA。这个名为pDM - 20的克隆包含了PLP的所有编码序列，但缺少编码PLP第117 - 151位氨基酸的105个碱基对。这种缺失会产生一种大小与DM - 20相同的蛋白质，DM - 20是中枢神经系统（CNS）髓磷脂的第二种主要蛋白脂蛋白。缺失5'端的DNA序列与供体剪接共有序列之间的同源性表明，PLP和DM - 20转录本是通过可变剪接从单个PLP基因衍生而来的。
