[紫、绿三七根茎中总花青素、总皂苷含量及皂苷单体组成]

[Contents of total anthocyanins and total saponins as well as composition of saponin monomers of Purple and Green Notoginseng Radix et Rhizoma].

作者信息

Zhao Chang-ling, Yang Sheng-chao, Chen Zhong-jian, Shen Yong, Wei Fu-gang, Wang Wu, Long Ting-ju

出版信息

Zhong Yao Cai. 2014 Oct;37(10):1749-53.

Abstract

OBJECTIVE

The contents of total anthocyanins and total saponins as well as the composition of saponin monomers of Purple and Green Notoginseng Radix et Rhizoma were studied to compare the medicinal quality and commercial values.

METHODS

Three-year-old Notoginseng Radix et Rhizoma was selected as the research materials. The contents of total anthocyanins and total saponins were determined by spectrophotometry. The compositions of saponin monomers were monitored by HPLC. The significance of content differences was determined by variance analysis.

RESULTS

The contents of total anthocyanins and total saponins of Purple Notoginseng Radix et Rhizomawere about 204.85% and 33.86% higher than those of Green Notoginseng Radix et Rhizoma respectively. The Purple and Green Notoginseng Radix et Rhizoma both contained five saponin monomers whose contents were as follows: ginsenoside Rg1 > ginsenoside Rb1 > notoginsenoside R1 > ginsenoside Rd > ginsenoside Re. The contents of notoginsenoside R1, ginsenoside Rd and ginsenoside Re of Purple Notoginseng Radix et Rhizoma were about 16.03%, 10.83% and 5.39% higher than those of Green Notoginseng Radix et Rhizoma respectively. However, the contents of ginsenoside Rg1 and ginsenoside Rb1 of Green Notoginseng Radix et Rhizoma were about 0.93% and 3.33% higher than those of Purple Notoginseng Radix et Rhizoma respectively. With respect to Green Notoginseng Radix et Rhizoma, the increase of the total anthocyanins in Purple Notoginseng Radix et Rhizoma reached a significant level, but the increases of total saponins, notoginsenoside R1, ginsenoside Rd and ginsenoside Re and the decreases of ginsenoside Rg1 and ginsenoside Rb1 did not.

CONCLUSION

The total anthocyanins accumulation in Notoginseng Radix et Rhizoma implies the content increases of the total saponins, notoginsenoside R1, ginsenoside Rd and ginsenoside Re, and the slight decreases of ginsenoside Rg1 and ginsenoside Rb1 contents; but the type and relative contents of saponin monomers remain unchanged. The medicinal quality and commercial value of Purple Notoginseng Radix et Rhizoma are higher than those of Green Notoginseng Radix et Rhizoma.

摘要

目的

研究紫、绿三七根茎中总花青素、总皂苷含量以及皂苷单体组成，比较二者药用品质及商业价值。

方法

选取三年生三七根茎作为研究材料。采用分光光度法测定总花青素和总皂苷含量。通过高效液相色谱法监测皂苷单体组成。采用方差分析确定含量差异的显著性。

结果

紫三七根茎中总花青素和总皂苷含量分别比绿三七根茎高约204.85%和33.86%。紫、绿三七根茎均含有5种皂苷单体，其含量依次为：人参皂苷Rg1＞人参皂苷Rb1＞三七皂苷R1＞人参皂苷Rd＞人参皂苷Re。紫三七根茎中三七皂苷R1、人参皂苷Rd和人参皂苷Re的含量分别比绿三七根茎高约16.03%、10.83%和5.39%。然而，绿三七根茎中人参皂苷Rg1和人参皂苷Rb1的含量分别比紫三七根茎高约0.93%和3.33%。对于绿三七根茎而言，紫三七根茎中总花青素的增加达到显著水平，但总皂苷、三七皂苷R1、人参皂苷Rd和人参皂苷Re的增加以及人参皂苷Rg1和人参皂苷Rb1的减少未达到显著水平。