光反应性寡核苷酸对RNA诱导沉默复合体的功能调控
Functional regulation of RNA-induced silencing complex by photoreactive oligonucleotides.
作者信息
Matsuyama Yohei, Yamayoshi Asako, Kobori Akio, Murakami Akira
机构信息
Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
出版信息
Bioorg Med Chem. 2014 Feb 1;22(3):1003-7. doi: 10.1016/j.bmc.2013.12.044. Epub 2013 Dec 27.
We developed a novel method for regulation of RISC function by photoreactive oligonucleotides (Ps-Oligo) containing 2'-O-psoralenylmethoxyethyl adenosine (Aps). We observed that inhibitory effects of Ps-Oligos on RISC function were enhanced by UV-irradiation compared with 2'-O-methyl-oligonucleotide without Aps. These results suggest Ps-Oligo inhibited RISC function by cross-linking effect, and we propose that the concept described in this report may be promising and applicable one to regulate the small RNA-mediated post-transcriptional regulation.
我们开发了一种通过含有2'-O-补骨脂素甲氧基乙基腺苷（Aps）的光反应性寡核苷酸（Ps-Oligo）来调节RISC功能的新方法。我们观察到，与不含Aps的2'-O-甲基寡核苷酸相比，紫外线照射增强了Ps-Oligos对RISC功能的抑制作用。这些结果表明，Ps-Oligo通过交联作用抑制RISC功能，并且我们提出本报告中描述的概念可能是一种有前景且适用于调节小RNA介导的转录后调控的方法。
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