分析 Macaranga tanarius 不同部位的抗氧化类异戊烯基黄酮，该植物是冲绳蜂胶的植物来源。

Analysis of antioxidant prenylflavonoids in different parts of Macaranga tanarius, the plant origin of Okinawan propolis.

机构信息

Department of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.

出版信息

Asian Pac J Trop Med. 2014 Jan;7(1):16-20. doi: 10.1016/S1995-7645(13)60184-4.

DOI:10.1016/S1995-7645(13)60184-4

PMID:24418076

Abstract

OBJECTIVE

To analyze the antioxidant prenylflavonoids in different parts of Macaranga tanarius (M. tanarius) (Euphorbiaceae) including the leaf, petiole, stem, leaflet, flower and fruit (only in female plant), and to evaluate their antioxidant properties.

METHODS

Methanol extracts of each part of M. tanarius were prepared and five prenylflavonoids in them were quantitatively analyzed using HPLC. The fruits from female plant were further separated into seed, pericarp, and glandular trichome. After the quantitative analyses of prenylflavonoids in each part of M. tanarius, antioxidant activity of the extracts was evaluated by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay.

RESULTS

The leaf of M. tanarius contained two prenylflavonoids as main components in both male and female plants. Both flowers (male and female) contained five kinds of prenylflavonoids. In the petiole, stem and leaflet of both male and female plants, the prenylflavonoids were not detected or their amounts were very low. Five kinds of prenylflavonoids were detected in the seed, pericarp and glandular trichome of female M. tanarius. In particular, the glandular trichome had the highest level of total prenylflavonoids (235 mg/g of fresh plant). DPPH radical scavenging activity of all parts was more than 30%.

CONCLUSIONS

We found that different parts of M. tanarius contained antioxidant prenylflavonoids. In particular, not only the glandular trichome but also the leaf contained prenylflavonoids, which indicated that M. tanarius may be developed as a functional plant, because the leaves of this plant can be easily collected.

摘要

目的

分析不同部位的大琴叶榕（Macaranga tanarius）（大戟科）中的抗氧化异戊烯基类黄酮，包括叶、叶柄、茎、小叶、花和果实（仅在雌性植物中），并评估其抗氧化特性。

方法

用高效液相色谱法对大琴叶榕各部位的甲醇提取物进行定量分析，定量分析了其中的五种异戊烯基类黄酮。雌性植物的果实进一步分离为种子、果皮和腺毛。对大琴叶榕各部位异戊烯基类黄酮进行定量分析后，用 2,2-二苯基-1-苦基肼（DPPH）自由基清除法评价提取物的抗氧化活性。

结果

大琴叶榕的叶在雄性和雌性植物中均含有两种异戊烯基类黄酮作为主要成分。两性花均含有五种异戊烯基类黄酮。在雄性和雌性植物的叶柄、茎和小叶中，未检测到异戊烯基类黄酮或其含量很低。在雌性大琴叶榕的种子、果皮和腺毛中检测到五种异戊烯基类黄酮。特别是腺毛总异戊烯基类黄酮含量最高（新鲜植物 235mg/g）。所有部位的 DPPH 自由基清除活性均大于 30%。