Kaneria Mital, Kanani Bhavana, Chanda Sumitra
Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot-360 005, Gujarat, India.
Asian Pac J Trop Biomed. 2012 Mar;2(3):195-202. doi: 10.1016/S2221-1691(12)60041-0.
To assess the effects of extraction methods on antioxidant activities of selected Indian medicinal flora.
Different parts of plants were extracted by hydroalcoholic and decoction methods using water and various concentrations of methanol (ME) viz. 75%, 50% and 25% ME. The antioxidant activity of all the different extracts was evaluated using two different antioxidant assays viz. 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging assay and superoxide anion radical scavenging assay. Total phenol and flavonoid content was also estimated.
The results showed that the extracting solvent significantly altered the antioxidant property estimations of screened plants. High correlations between phenolic compositions and antioxidant activities of extracts were observed. High levels of antioxidant activities were detected in Manilkara zapota (M. zapota) as compared with other screened plants.
The results obtained appear to confirm the effect of different methods on extraction of antioxidants and antioxidant property of M. zapota.
评估提取方法对所选印度药用植物抗氧化活性的影响。
采用水和不同浓度甲醇（75%、50%和25%甲醇）的水醇法和煎煮法提取植物的不同部位。使用两种不同的抗氧化测定法，即2,2-二苯基-1-苦基肼（DPPH）自由基清除测定法和超氧阴离子自由基清除测定法，对所有不同提取物的抗氧化活性进行评估。还估计了总酚和黄酮含量。
结果表明，提取溶剂显著改变了所筛选植物的抗氧化性能评估。观察到提取物的酚类成分与抗氧化活性之间存在高度相关性。与其他筛选植物相比，人心果（Manilkara zapota）中检测到高水平的抗氧化活性。
所得结果似乎证实了不同方法对人心果抗氧化剂提取及抗氧化性能的影响。
