Swenberg M L, Buck S H, Lovenberg W
Brain Res. 1987 Aug 4;417(1):131-8. doi: 10.1016/0006-8993(87)90187-9.
Polyclonal antibodies against substance P were raised in rabbits and partially purified. This apparently homogeneous immunoglobulin fraction was used to immunize other rabbits. The anti-idiotypic antibodies derived from these rabbits were substantially more effective in competing with substance P than in competing with beta-endorphin for binding to their respective primary antibodies. The anti-idiotypic antibody was also 50 times more potent in competing with substance P binding than in competing with substance K binding to rat duodenal membranes, a tissue containing receptors for substance P and substance K. The anti-idiotypic antibodies exhibited significant enhancement of substance P induced spasmogenic response on the rat uterus and guinea pig ileum (GPI). The results indicate that it is possible to develop anti-idiotypic antibodies that recognize substance P receptors. These antibodies will be of value in studies of the physiological roles of the neuropeptide, substance P.
针对P物质的多克隆抗体在兔体内产生并部分纯化。这个明显均一的免疫球蛋白组分被用于免疫其他兔子。源自这些兔子的抗独特型抗体在与P物质竞争结合其各自的一级抗体方面，比与β-内啡肽竞争结合更有效。抗独特型抗体在与P物质结合竞争方面的效力也比与物质K结合竞争高50倍，物质K可与大鼠十二指肠膜结合，该组织含有P物质和物质K的受体。抗独特型抗体在大鼠子宫和豚鼠回肠（GPI）上对P物质诱导的致痉挛反应有显著增强作用。结果表明，有可能开发出识别P物质受体的抗独特型抗体。这些抗体在研究神经肽P物质的生理作用方面将具有价值。
