Couraud J Y, Maillet S, Conrath M, Calvino B, Pradelles P
Département de Biologie, CEN Saclay, Gif sur Yvette, France.
Mol Chem Neuropathol. 1990 Jan;12(1):71-82. doi: 10.1007/BF03160058.
In order to develop immunological tools for studying the receptor of the neuropeptide substance P (SP), anti-idiotypic antibodies (anti-Id abs) were produced by immunization with anti-SP antibodies whose specificity was close to that of the SP receptor. Immunological studies revealed structural similarity between some anti-Id abs and SP itself. As a consequence, these anti-Id abs were able to bind to mammalian SP receptors. These antibodies were used in immunocytochemistry to label SP receptors both in the rat spinal cord and in rat and guinea pig peripheral tissues (parotid gland and trachea, respectively). Like SP, anti-Id abs were able to trigger protein secretion by isolated rat parotid gland cells. Finally, it was shown that anti-Id abs in vivo modulated reactivity to chemical stimuli. These antibodies therefore appear to be promising tools for further biochemical, cytochemical, and pharmacological characterization of SP receptors.
为了开发用于研究神经肽P物质（SP）受体的免疫学工具，用特异性与SP受体相近的抗SP抗体进行免疫，制备了抗独特型抗体（抗Id抗体）。免疫学研究揭示了一些抗Id抗体与SP自身之间的结构相似性。因此，这些抗Id抗体能够与哺乳动物SP受体结合。这些抗体被用于免疫细胞化学，以标记大鼠脊髓以及大鼠和豚鼠外周组织（分别为腮腺和气管）中的SP受体。与SP一样，抗Id抗体能够触发分离的大鼠腮腺细胞的蛋白质分泌。最后，研究表明抗Id抗体在体内可调节对化学刺激的反应性。因此，这些抗体似乎是对SP受体进行进一步生化、细胞化学和药理学特性鉴定的有前途的工具。
